先進螢光與多光子顯微技術
Advanced fluorescence and multiphoton microscopy techniques
| 節 | 週三 |
|---|---|
A 18:30–19:20 | 先進螢光與多光子顯微技術 2 節連堂 |
B 19:30–20:20 |
* 根據陽明交大上課時間表所列
Microscopy background and techniques; resolution and image formation; fluorescence and phosphorescence; important dyes, detectors and lasers for ultrashort pulses; microscopic imaging of biological samples; confocal imaging laser scanning principle; multiphoton imaging; non-linear Raman imaging; superresolution techniques; light sheet microscopy; insight into clinical applications including regulations.
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教學方法及教學相關配合事項(如助教、網站或圖書及資料庫等) On completion of the course, students shall be able to; 1. explain basic microscopic image formation; 2. understand the concept of point spread function and resolution; 3. understand technical setups for fluorescence imaging; 4. explain the principles of nonlinear excitation and its use for multiphoton microscopy; 5. understand the characteristics of ultrashort lasers and light detectors; 6. understand the special regulations for clinical applications and requirements for lab-to-clinic transfer of laser imaging devices; 7. comprehend superresolution techniques including capabilities and limitations.
1.學期作業、考試、評量 Homework, Exam, and Evaluation
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| 週次 | 主題 |
|---|---|
| 第 1 週 | Microscopy: Introduction and Overview |
| 第 2 週 | Optics background: lenses and more |
| 第 3 週 | Compound microscope: Abbe, Airy, and Rayleigh |
| 第 4 週 | Fluorescence fundamentals and general fluorescence microscopy |
| 第 5 週 | Confocal and laser scanning microscopy |
| 第 6 週 | Lasers for imaging and how to make really short pulses |
| 第 7 週 | Multiphoton microscopy |
| 第 8 週 | Mid-term exam |
| 第 9 週 | Lifetime imaging: FLIM and PLIM |
| 第 10 週 | Raman Scattering and Imaging |
| 第 11 週 | CARS and nonlinear Raman Scattering methods |
| 第 12 週 | Clinical multimodal multiphoton microscopy |
| 第 13 週 | Medical Device Regulations and laser safety |
| 第 14 週 | Light sheet microscopy; Gaussian and Bessel lattices |
| 第 15 週 | Super resolution microscopy |
| 第 16 週 | Final exam |
| 第 17 週 | online course 1 |
| 第 18 週 | online course 2 |
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