腦科學中的分子工程
Molecular Engineering Tools in Brain Science
| 節 | 週三 |
|---|---|
3 10:10–11:00 | 腦科學中的分子工程 YL837（陽明） 2 節連堂 |
4 11:10–12:00 |
* 根據陽明交大上課時間表所列
本課程著重於探討近年分子工程的發展,並透過已發表的論文與實驗來瞭解這些新興的分子工具在腦科學研究上的應用。期待同學們在瞭解這些分子工具後,能對自己的研究有所啟發。 This course explores the cutting-edge advances in engineered molecular tools. We will examine research articles that showcase how these innovations are transforming our understanding of the brain. By gaining a deep appreciation of these engineered molecular tools, we hope to inspire the development of your own research.
基礎英語溝通能力 (Basic English communication skill)
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Paper presentation (70%) and in-class discussion (30%) 論文報告 (70%) 與課堂討論 (30%)
- Paper discussion
- Engineered molecular tools
| 週次 | 主題 |
|---|---|
| 第 1 週 | Basic proximity labeling enzymes |
| 第 2 週 | Advanced proximity labeling enzymes |
| 第 3 週 | Engineered cAMP sensors |
| 第 4 週 | The application of PKA sensors |
| 第 5 週 | Calcium sensors |
| 第 6 週 | Genetically encoded voltage indicators |
| 第 7 週 | Genome-editing toolbox |
| 第 8 週 | Engineered Cas9 proteins |
| 第 9 週 | Engineered mechano-sensors |
| 第 10 週 | Advanced toolbox for protein-protein interactions |
| 第 11 週 | The applications of chimera proteins |
| 第 12 週 | DNA and protein delivery |
| 第 13 週 | Multifaceted applications of antibodies |
| 第 14 週 | Image-based brain connectomes |
| 第 15 週 | Presentation and Discussion |
| 第 16 週 | Presentation and Discussion |
1. Molecular Cloning: A Laboratory Manual (Fourth Edition) By Michael R. Green, and Joseph Sambrook, 2012, Cold Spring Harbor Laboratory Press 2. Protein Engineering: Tools and Applications By Huimin Zhao, Sang Yup Lee, Jens Nielsen, Gregory Stephanopoulos First published:6 August 2021, Wiley Online Library
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